The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Abstract Summary

Below is a summary of the abstract you submitted. Presenting author(s) is shown in bold.

If any changes need to be made, you can modify the abstract or change the authors.

You can also download a .docx version of this abstract.

If there are any problems, please email Dan at and he'll take care of them!

This abstract was last modified on April 26, 2018 at 11:45 a.m..

Iowa State University
Corresponding Faculty Member: Nick Peters,
This abstract will NOT be considered for a talk.
Identification and Characterization of mycobacteriophage Olga
Inna Couri, Gretchen Swift, Nick Peters, Nancy Noury

Olga was isolated by Inna Couri, and is a cluster G1 phage. It was discovered in a flower pot on campus at Iowa State University by direct isolation using Mycobacterium smegmatis as a host. This phage has a genome that is 41902 bases in length and a CG content of 66.6%, and transmission electron microscopy revealed the morphotype as Siphoviridae. Initial Blast analysis of the genome showed that phages Annihilator and Avrafan were most similar. Annotation of Olga’s genome indicated that there were 62 genes and no transfer RNAs. While working with the phage it explicitly expressed the lytic life cycle, but as the genome was annotated we found that under certain conditions this phage could possibly express a lysogenic cycle due to genes particular to the lysogenic process. There were also additional genes that made Olga unique from its closest relatives, which revealed some potentially interesting evolutionary events in progress for this phage. Upon completion of annotation we were not able to assign a function to every gene that we identified, which is typical for phage genomes. Though this phage has many close relatives found in the database, here we describe some interesting elements that set Olga apart from its relatives.